site stats

Stat6 reporter assay

WebThe STAT6 Leeporter™ Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the … WebAssay Validation Multiple analytes being measured? The analytical performance of each analyte must be evaluated to ensure that the method is sufficient for use in analysis of all …

Stat6 inhibits human interleukin-4 promoter activity in T cells

WebApr 11, 2024 · We have observed that STAT6 was phosphorylated at Y641 and serine. Tyrosine phosphorylation is independent of JNK-1, while serine phosphorylation is dependent on the aforementioned kinase. ... This controversy intensified when the same authors used luciferase-reporter-based assays to show that the S407 mutation nullifies … WebJan 31, 2024 · The STAT6 inhibitor AS1517499 was used to specifically inhibit p-STAT6. The effect of p-STAT6 inhibition on Ph+ CML and Ph+ ALL cells was identified by CCK-8 and FCM assay. Dual luciferase reporter and ChIP assay were performed to confirm the direct binding between STAT6 and c-Myc. mobile organic biofilm wastewater https://all-walls.com

HEK-Blue IL-4/IL-13 Cells IL-4 and IL-13 Reporter Cells

WebDescription. The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase … WebApr 14, 2024 · The STAT6 Leeporter™ Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional … WebDec 10, 2024 · Beta actin was used as internal control. (F) Reporter assays of STAT6 promoter. HEK293 cells co-transfected STAT6 promoter-driven luciferase reporter with different dosage RTA (0, 1, 5, 10μg) were subjected to reporter assay. Relative firefly luciferase unit (RLU) normalization with Renilla activity was analyzed. Data is presented … mobile organised crime gangs

Structural basis for DNA recognition by STAT6 PNAS

Category:IL-4 & IL-13 Reporter HEK 293 Cells - InvivoGen

Tags:Stat6 reporter assay

Stat6 reporter assay

STAT6 degradation and ubiquitylated TRIML2 are essential for

WebSTAT6-dependent SEAP activity is readily assessable in the supernatant using QUANTI-Blue™ Solution, a detection reagent. STING Background STING (stimulator of interferon … InvivoGen reporter cells are designed to monitor the activation of intracellular … These cell lines express different isoforms of the Dectin-1 gene. They also express … STING ligand 2’3’-cGAMP is a natural cyclic di-nucleotide produced in mammalian … Cell Culture. InvivoGen provides a wide range of high-quality reagents for tissue … HEK-Blue™ STAT6-hSTING-R232 cells Back to the top. Contents. ... Human TLR2 / NF … 2’3’-c-di-AM(PS)2 (Rp,Rp) is a synthetic analog of natural 3’3’-c-di-AMP. It … Specifications. Product concentration: 100 mg/ml solution CAS number: 31282-04-9 … WebMay 15, 2024 · Dual-luciferase reporter assay. Targetscan was used to predict the up-stream regulatory gene of STAT6, and miR-135a-3p was proved to regulate STAT6. ... The viability of RA FLSs transfected with si-STAT6 was assessed by CCK-8 assay at indicated times; (B) The proliferation of RA FLSs transfected with si-STAT6 was evaluated by EdU …

Stat6 reporter assay

Did you know?

WebJul 18, 2024 · Reporter assays showed increased IL-4 induced transactivation activity of STAT6 MUT at the PARP14 promotor, suggesting a self-reinforcing regulatory circuit. Knock-down of PARP14 or... WebOct 24, 2024 · When transfecting STAT6-expressing plasmid pLS16 (P hCMV-STAT6-pA) and reporter plasmid pLS12 ... The histamine release assay was performed in an incubator at 37 °C in a humidified 5% CO2-containing atmosphere for 30 min. Thereafter, the cells were centrifuged for 10 min at 700 ...

WebJan 31, 2024 · Dual luciferase reporter and ChIP assay were performed to confirm the direct binding between STAT6 and c-Myc. The impact of STAT6 inhibition on tumor progression was detected in Ph+ CML and Ph+ ALL mouse models. Results Our results demonstrated that P210 induced CML-like disease, and P190 caused the more malignant ALL-like … WebDescription. The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection …

WebJan 22, 2015 · Luciferase reporter assays on an extended panel of naturally occurring genomic STAT6 sites derived from FCER2, CISH, NFIL3, CCL17, and AGAP9 demonstrated … WebDec 15, 1998 · We report here that Stat6 interacts with three binding sites in the human IL-4 promoter by electrophoretic mobility shift assays. These sites overlap the P1, P2, and P4 …

WebSTAT Reporter Cell Line - Tool for Screening of Drugs Targeting JAK/STAT Pathway Signal transducer and activator of transcription (STAT) protein is a family of transcription factors …

WebThe results from the reporter assays strongly suggested that PU.1 facilitates Stat6‐mediated transcription by directly binding to its core binding sequence in the DNA within the Stat6 response element on the Igϵ promoter. However, it was necessary to investigate whether the point mutation affected the DNA‐binding of Stat6. mobile originated smsWebApr 12, 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ... mobile or manufactured homes warehouseWebCyclooxygenase-2 (COX-2) is frequently overexpressed in human cancers and contributes to the malignant phenotype. Our data indicate unphosphorylated signal transducers and activators of transcription mobile osha testing cleveland