Isg54 promoter
WebOct 31, 2014 · Abstract. The σ 54 promoters are unique in prokaryotic genome and responsible for transcripting carbon and nitrogen-related genes. With the avalanche of … WebThe ISG54 ISRE oligonucleotide spans from −75 to −114 of the ISG54 promoter. The HLA-B7 ISRE oligonucleotide spans from −109 to −128 of the HLA-B7 promoter. The sequences of the HLA-A2; HLA-B7; and ISG54 M1, …
Isg54 promoter
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WebThe isoflavone compounds activated the ISG54 promoter, mediated nuclear translocation of IRF-3, and displayed highly potent activity against hepatitis C virus (HCV) and influenza virus. Additionally, these agonists efficiently activated IRF-3 in the presence of the HCV protease NS3-4A, which is known to blunt the host immune response.
WebJul 24, 2012 · Overall fold of ISG54. Figure 1 and Table 1). Most residues of the protein, except for the first 5 residues at the N-terminus, the last 22 residues at the C-terminus and … WebNational Center for Biotechnology Information
Webwith the promoter of the IFN / -stimulated gene ISG54 (3–6). From these observations one would predict that incubation of cells with HDAC inhibitors would increase IFN-activated gene expression, and this is indeed the case when one examines IFN -stimulated expression of the MHC-II (7). However, in contrast to IFN , interleukin-3-activated ... WebMar 4, 2011 · Expression of ISG54, independent of IFN stimulation, elicits apoptotic cell death. Cell death and apoptosis were quantified by propidium iodide uptake and annexin-V staining, respectively. The activation of caspase-3, a key mediator of the execution phase of apoptosis, was clearly apparent in cells expressing ISG54.
WebThis composite promoter is comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-kB or …
WebISG54 promoter activity was significantly reduced in IRF3KO RAW264.7 cells responding to IFN-γ, poly I:C, or IFN-γ plus poly I:C, compared with WT RAW264.7 cells. These data were … horse in field of peonies photographyWebMar 1, 1994 · The interferon-alpha(IFN-alpha)-regulated hamster ISG-54 K gene, which is activated in hamster CHO-12 cells at least 40-fold, was isolated and the promoter region … ps4 nfoWebDec 17, 2024 · In this study, we found BFRF1 could down-regulate sendai virus-induced IFN-β promoter activity and mRNA expression of IFN-β and ISG54 during BFRF1 plasmid transfection and EBV lytic infection, but BFRF1 could not affect the promoter activity of NF-κB or IRF7. Specifically, BFRF1 could co-localize and interact with IKKi. horse in field of flowersWebAt indicated times, (A) luciferase activity in 10 μL of supernatants was used to determine relative ISG54 promoter activity or (B) ISG54 expression was determined using qRT-PCR or (C) protein lysates were collected and ISG54 and β-Tubulin (β-Tub) were evaluated by western blot. (A) two independent experiments, each with n=3. (B) ... ps4 nfl themesWebRAW-Lucia™ ISG-KO-STING cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE. RAW 264.7 have been reported to express several CDSs, including cGAS [1]. RAW-Lucia™ ISG-KO-STING cells allow the monitoring of IRF ... horse in forest paintingWebsimilarity in opposite orientations in the ISG54 and ISG15 promoters (5, 6). Similar sequences have been found in promoter regions of all other IFN-a/,8-stimulated genes described so far (5-10). This conserved sequence, which constitutes an IFN-stimulated response element (ISRE), was protected in DNase I footprint experiments with crude ps4 next month free gamesWebDownload scientific diagram Identification of small-molecule inducers of the ISG54 promoter. (A) A 20,000-member diversity library was screened at 10 M to identify compounds (Cpnd.) that ... ps4 next to ps5