WebHILIC Mixed-Mode Separation of Neutral, Acidic and Basic Sugars Choosing the right column and mobile phase guarantees the robustness of analytical method. Knowing how to control retention times of ... WebAug 15, 2013 · It is also important to notice that the ion-exchange mechanism, always present with HILIC phases, plays a major role for the retention of peptides. Based on my own experience, I would indicate that 95% of peptides can be adequately analysed in HILIC thanks to the ion-exchange contribution.
Hydrophilic Interaction Chromatography - an overview
Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was … WebMay 12, 2024 · Ultisil® HILIC Amphion II Column. Ultisil® HILIC Amphion II is a newly developed HILIC column, using amphion-bonded silica as packing material. ... Charge Centre and Negative Charge Centre, brings high retention for acid and alkaline compounds through ion-exchange mechanism. Compared with common HILIC packing materials like silica … graph and tree difference in data structure
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WebDec 2, 2015 · For this reason, hydrophilic interaction chromatography (HILIC) separation mechanisms have been used extensively as an alternative separation mode, spurring the development of zwitterionic … WebHILIC or hydrophilic interaction liquid chromatography is a high-performance liquid chromatographic (HPLC) technique for separation of polar and hydrophilic compounds. Simply put, HILIC is a normal-phase type of separation but uses reversed-phase type eluents. Web•HILIC is a variation of normal-phase chromatography •Stationary phase is a polar material such as silica, cyano, amide, etc. •A high organic-low aqueous mobile phase •i.e. water is the elution solvent •Mechanism is a combination of weak cation exchange and partitioning into the adsorbed aqueous layer chipshed.co.uk