WebJun 1, 2024 · The restriction digestions were performed using the EpiTect Methyl II DNA Restriction Kit provided by Qiagen (Chatsworth, CA, USA) as previously described [19]. The digested DNA samples were then analyzed using the Human Inflammatory Response and Autoimmunity EpiTect Methyl II Signature PCR Array Profiles (Qiagen), as … WebEpiTect PCR Control DNA Set (100) Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs $461.00 Add to …
EpiTect Bisulfite Kits - Qiagen
WebPCR Enzymes & Kits. End-Point PCR; HiFidelity, Long-Range & Other PCR; One-Step RT-PCR; ... DNA Methylation Analysis; Metagenomics. Whole Genome Metagenomics; … WebNov 1, 2016 · DNA was eluted in nuclease-free water before digestion using the EpiTect® Methyl II DNA Restriction Kit (Qiagen) according to the manufacturer’s recommendations. Samples were stored at −20°C. The methylation profile was analysed using the EpiTect® Methyl II Signature PCR Array Human DNA Repair kit (Qiagen) according to the … merrill lynch commercial actress 2023
EpiTect Methyl qPCR Assay Handbook - qiagen.com
WebFigure Legend Snippet: Effect of EGCG on global DNA methylation in HeLa cells a The global methylation level of DNA in HeLa cells treated with 50 µM EGCG for 24 h and 48 h was assessed using an ELISA-based assay. DNA from untreated HeLa cells were used as control. EGCG significantly decreased the levels of global DNA methylation in HeLa … WebDec 18, 2014 · If using the EpiTect Methyl II DNA Restriction Kit, use 26 μL of 5× Digestion Buffer and add dH 2 O to a final volume of 120 μL. If using restriction enzymes from another source, prepare a volume of 200 μL, which is sufficient for four 50 μL individual restriction digest reactions ( see Note 2 ). how sbp works