Ctcf kda

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August undefined, 2024

WebJan 30, 2024 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific … WebThe recombinant protein with a predicted molecular weight of 43.3 kDa was removed from the gel and purified by electroelution. The electroelution was conducted in cellulose tubes (Sanko Junyaku Co., Ltd., Tokyo, Japan) filled with an electroelution buffer (100 mM sodium phosphate and 0.1% SDS; pH 7.0) at 100 V and 100 mA for 17 h.

Decreased poly(ADP-ribosyl)ation of CTCF, a transcription

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Anti-CTCF Antibody 07-729 - EMD Millipore

WebJul 10, 2024 · Silver-stained SDS PAGE gel of IP proteins showing chicken CTCF protein of approximately 140 kDa, and lamprey CTCF protein of >250 kDa. Detailed procedures of western blotting and ... WebCUT&RUN was performed on 500k K562 cells with either CTCF (0.125 µg) or IgG negative control (0.5 µg, EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v3.0 (EpiCypher 14-1048).Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit … WebApr 10, 2024 · It contains 37 exons that encode a 2492 amino acid protein with a molecular weight of 282.586 kDa [22,23]. Based on its ATPase/helicase C-terminal domain, the protein is a member of the SWI/SNF family. ... (CTCF, which is localized by H3.3 histones) and boundary disturbances of the chromatin loop, which promote coordinated loop-wide … earned income credit 2020 calculator

Decreased poly(ADP-ribosyl)ation of CTCF, a transcription

WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. WebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human … csv python headerWebApr 6, 2012 · Here, we report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses. We found that stimulation of human corneal … earned income credit 2020 chart pdf

"WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. " - Ctcf kda

Ctcf kda

RCSB PDB - 5KKQ: Homo sapiens CCCTC-binding …

WebJun 8, 2004 · The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5' end of the chicken beta-globin locus. ... a 38-kDa nucleolar phosphoprotein that is concentrated in nuclear matrix preparations. In this ... WebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific targets of poly(ADP-ribosyl)ation in tumor development. Two forms of the multifunctional transcription factor, CTCF, were previously identified: a 130-kDa protein (CTCF-130), …

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WebFeb 12, 2024 · CTCF expression in neural stem cells (NSCs) or neurons is essential for fly viability. To identify a biologically relevant tissue in which to study CTCF function in Drosophila, we used previously ... WebMar 3, 2024 · The shorter CTCF forms are expected to lack several domains; in the ~ 120 kDa form, we estimate that most of the N-terminal part that is responsible for cohesin retention and formation of genomic loops (Pugacheva et al. 2024) is missing, but all eleven zinc-finger still exist.

WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. Web17.9 kDa No: 0: CTCF-212 ENSP00000495218 ENST00000645306: A0A2R8YFL0 [Direct mapping] Transcriptional repressor CTCF . Show all. Predicted intracellular proteins Intracellular proteins predicted by MDM and MDSEC Cancer-related genes Mutated cancer genes Mutational cancer driver genes

WebFeb 1, 1997 · CTCF belongs to the Zn finger transcription factors family and binds to the promoter region of c-myc. CTCF is highly conserved between species, ubiquitous and localised in nuclei. The endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a … WebAug 17, 2024 · CTCF is required for peripheral nerve ensheathment a Excised exon 8 of the floxed Ctcf allele by Dhh-Cre. b Co-labeling of CTCF with SOX10 in control and mutant sciatic nerves at P7 (n = 3 animals ...

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WebGeneral description. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator ... earned income credit 2020 income limitWebCTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to … csv python 書き込み辞書WebMay 1, 2006 · Interestingly, the 180 kDa poly(ADP-ribosyl)ated isoform of CTCF was predominantly found in the nucleoli fractions. By transfecting different CTCF deletion constructs into cell lines of different origin we demonstrate that the central zinc-finger domain of CTCF is the region responsible for nucleolar targeting. Analysis of … csv python write list to fileWebMar 11, 2014 · CTCF creates boundaries between topologically associating domains in chromosomes and, within these domains, facilitates interactions between transcription … csv python read_csvhttp://www.zen-bioscience.com/prod_view.aspx?IsActiveTarget=True&TypeId=163&Id=430151&FId=t3:163:3 csv python pandas 書き込みWebMay 23, 2016 · The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We … csv python pandas 読み込みWebAbFlex® CTCF recombinant antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of MCF7 chromatin and 4 µg of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to … earned income credit 2020 qualifications